Alpha-1 Microglobulin
A-1 Microglobulin
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Cat. No.:
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KAI-056
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Type:
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Reagent
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Method:
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Immunoturbidimetric
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Format:
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Liquid
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Linearity:
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Serum/Plasma: 1.0 - 137.0 mg/L (or value of highest calibration point)
Urine: 0.2 - 34.0 mg/L (or value of highest calibration point)
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Sample:
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Serum, Plasma, Urine
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KIT COMPOSITION
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INTENDED USE
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For the quantitative determination of human Alpha-1 Microglobulin in human
urine, serum, and plasma by immunoturbidimetric assay.
FOR IN VITRO DIAGNOSTIC USE.
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REAGENT PREPARATION
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The reagents are ready to use and do not require reconstitution.
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STORAGE AND HANDLING
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All reagents should be stored refrigerated (2-8°C). Return all reagents to
2-8°C promptly after use. Unopened reagents can be used for one year from the
date of manufacture, as indicated by the expiration date on the package and bottle
labels. Opened reagents can be used for one month if stored at 2-8°C. It
is recommended that R-2 be gently inverted once a week after it is opened.
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PERFORMANCE
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Precision
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Precision - Within Run
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Serum
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Sample I
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Sample II
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Sample III
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N = 10
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N = 10
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N = 10
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Mean = 7.1
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Mean = 32.1
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Mean = 66.1
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S.D. = 0.145
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S.D. = 0.263
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S.D. = 0.540
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C.V. = 2.04%
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C.V. = 0.82%
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C.V. = 0.82%
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Urine
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Sample I
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Sample II
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Sample III
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N = 10
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N = 10
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N = 10
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Mean = 0.7
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Mean = 3.3
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Mean = 17.4
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S.D. = 0.029
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S.D. = 0.012
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S.D. = 0.059
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C.V. = 3.93%
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C.V. = 0.37%
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C.V. = 0.34%
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Precision - Between Runs
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Serum
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Sample I
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Sample II
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Sample III
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N = 15
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N = 15
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N = 15
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Mean = 7.5
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Mean = 32.3
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Mean = 67.1
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S.D. = 0.15
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S.D. = 0.31
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S.D. = 1.27
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C.V. = 2.0%
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C.V. = 0.9%
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C.V. = 1.9%
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Urine
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Sample I
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Sample II
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Sample III
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N = 15
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N = 15
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N = 15
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Mean = 0.72
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Mean = 3.3
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Mean = 17.2
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S.D. = 0.046
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S.D. = 0.04
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S.D. = 0.31
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C.V. = 6.3%
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C.V. = 1.2%
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C.V. = 1.8%
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Accuracy / Correlation
A comparison of the
K-ASSAY ®
Alpha-1 Microglobulin assay and the latex agglutination method of another company is given below:
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Serum samples:
y = 1.00x + 2.83
r = 1.00
n = 55
x = Company A's latex reagent
y = K-ASSAY ® Alpha-1 Microglobulin assay
Urine samples:
y = 1.00x - 0.52
r = 1.00
n = 55
x = Company A's latex reagent
y = K-ASSAY ® Alpha-1 Microglobulin assay
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Assay Range
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Serum/Plasma: 1.0 - 137.0 mg/L (or value of highest calibration point)
Urine: 0.2 - 34.0 mg/L (or value of highest calibration point)
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INTERFERENCE
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Bilirubin F and C :
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No interference up to 20 mg/dL
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Hemoglobin :
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No interference up to 500 mg/dL
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Lipemia :
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No interference up to 5%
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