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Transferrin
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Cat. No.:
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KAI-023
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Type:
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Reagent
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Method:
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Immunoturbidimetric
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Format:
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Liquid
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Linearity:
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45 - 455 mg/dL
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Sample:
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Serum
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KIT COMPOSITION
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INTENDED USE
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The K-ASSAY ®
Transferrin assay is intended for the quantitative determination of human transferrin in human serum by immunoturbidimetric assay.
FOR IN VITRO DIAGNOSTIC USE.
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REAGENT PREPARATION
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The reagents are ready to use and do not require reconstitution.
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STORAGE AND HANDLING
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All reagents should be stored refrigerated (2-8°C). Return all reagents to
2-8°C promptly after use. Unopened reagents can be used for one year from the
date of manufacture, as indicated by the expiration date on the package and bottle
labels. Opened reagents can be used for one month if stored at 2-8°C.
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PERFORMANCE
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Precision
The precision for the
K-ASSAY ®
Transferrin assay was determined using packaged reagents, pooled human serum, and a Hitachi 717 analyzer.
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Precision - Within Run
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Sample I
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Sample II
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Sample III
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N = 20
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N = 20
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N = 20
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Mean = 70.7
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Mean = 300.75
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Mean = 526.4
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S.D. = 0.92
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S.D. = 5.02
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S.D. = 5.77
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C.V. = 1.3%
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C.V. = 1.67%
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C.V. = 1.097%
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Precision - Between Runs
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Sample I
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Sample II
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N = 8
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N = 8
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Mean = 175.38
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Mean = 340.9
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S.D. = 1.6
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S.D. = 1.46
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C.V. = 0.91%
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C.V. = 0.4%
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Accuracy / Correlation
A comparison of the
K-ASSAY ®
Transferrin assay to an Incstar Transferrin Test Kit was performed using a Hitachi 717. The test results provided the following data:
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y = 1.064x - 50.124
r = 0.982
n = 60
x = Incstar Transferrin Test Kit
y = K-ASSAY ® Transferrin
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x
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min
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=
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148
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y
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min
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=
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110
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max
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=
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440
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max
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=
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426
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mean
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=
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246
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mean
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=
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212
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Assay Range
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INTERFERENCE
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Ascorbic Acid :
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No interference up to 50 mg/dL
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Bilirubin C :
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No interference up to 20 mg/dL
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Bilirubin F :
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No interference up to 20 mg/dL
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Hemoglobin :
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No interference up to 500 mg/dL
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Intralipid :
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No interference up to 500 mg/dL
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