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Complement C3
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Cat. No.:
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KAI-009
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Type:
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Reagent
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Method:
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Immunoturbidimetric
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Format:
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Liquid
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Linearity:
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30 - 350 mg/dL
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Sample:
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Serum
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KIT COMPOSITION
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INTENDED USE
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For the quantitative determination of human complement C3 (3rd complement component) in serum by
immunoturbidimetric assay. Complement is a group of serum proteins that destroy infectious
agents. Measurement of these proteins aids in the diagnosis of immunological disorders,
especially those associated with deficiencies of complement components.
FOR IN VITRO DIAGNOSTIC USE.
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REAGENT PREPARATION
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The reagents are ready to use. They do not need to be reconstituted.
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STORAGE AND HANDLING
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All reagents should be stored refrigerated (2-8°C). Return all reagents to
2-8°C promptly after use. Unopened reagents can be used for one year from the
date of manufacture, as indicated by the expiration date on the package and bottle
labels. Opened reagents can be used for one month if stored at 2-8°C.
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PERFORMANCE
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Precision
The precision for the
K-ASSAY ®
Complement C3 assay was determined using packaged reagents, pooled human serum, and a Hitachi 717 analyzer.
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Precision - Within Run
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Sample I
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Sample II
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Sample III
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N = 20
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N = 20
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N = 20
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Mean = 33.7
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Mean = 75.6
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Mean = 112.4
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S.D. = 1.12
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S.D. = 1.68
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S.D. = 2.18
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C.V. = 3.33%
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C.V. = 2.22%
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C.V. = 1.94%
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Precision - Between Runs
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Sample I
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Sample II
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Sample III
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N = 10
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N = 10
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N = 10
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Mean = 35.4
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Mean = 84.3
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Mean = 114.4
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S.D. = 1.29
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S.D. = 1.95
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S.D. = 1.70
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C.V. = 3.63%
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C.V. = 2.31%
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C.V. = 1.48%
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Accuracy / Correlation
A comparison of the
K-ASSAY ®
Complement C3 assay and an INCSTAR Complement C3 Test Kit was performed using a Hitachi 717. The test results provided the following data:
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y = 0.842x + 4.727
r = 0.925
n = 60
x = INCSTAR Complement C3 Test Kit
y = K-ASSAY ® Complement C3 assay
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x
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min
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=
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78
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y
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min
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=
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74
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max
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=
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188
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max
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=
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171
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mean
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=
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135
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mean
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=
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118
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Assay Range
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INTERFERENCE
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Bilirubin C :
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No interference up to 20 mg/dL
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Bilirubin F :
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No interference up to 20 mg/dL
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Hemoglobin :
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No interference up to 500 mg/dL
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Intralipid :
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No interference up to 500 mg/dL
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